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Issue 5, 2014
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Exposure of vitamins to UVB and UVA radiation generates singlet oxygen

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Abstract

Deleterious effects of UV radiation in tissue are usually attributed to different mechanisms. Absorption of UVB radiation in cell constituents like DNA causes photochemical reactions. Absorption of UVA radiation in endogenous photosensitizers like vitamins generates singlet oxygen via photosensitized reactions. We investigated two further mechanisms that might be involved in UV mediated cell tissue damage. Firstly, UVB radiation and vitamins also generate singlet oxygen. Secondly, UVB radiation may change the chemical structure of vitamins that may change the role of such endogenous photosensitizers in UVA mediated mechanisms. Vitamins were irradiated in solution using monochromatic UVB (308 nm) or UVA (330, 355, or 370 nm) radiation. Singlet oxygen was directly detected and quantified by its luminescence at 1270 nm. All investigated molecules generated singlet oxygen with a quantum yield ranging from 0.007 (vitamin D3) to 0.64 (nicotinamide) independent of the excitation wavelength. Moreover, pre-irradiation of vitamins with UVB changed their absorption in the UVB and UVA spectral range. Subsequently, molecules such as vitamin E and vitamin K1, which normally exhibit no singlet oxygen generation in the UVA, now produce singlet oxygen when exposed to UVA at 355 nm. This interplay of different UV sources is inevitable when applying serial or parallel irradiation with UVA and UVB in experiments in vitro. These results should be of particular importance for parallel irradiation with UVA and UVB in vivo, e.g. when exposing the skin to solar radiation.

Graphical abstract: Exposure of vitamins to UVB and UVA radiation generates singlet oxygen

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Publication details

The article was received on 02 Dec 2013, accepted on 27 Feb 2014 and first published on 04 Mar 2014


Article type: Paper
DOI: 10.1039/C3PP50413A
Citation: Photochem. Photobiol. Sci., 2014,13, 820-829
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    Exposure of vitamins to UVB and UVA radiation generates singlet oxygen

    A. Knak, J. Regensburger, T. Maisch and W. Bäumler, Photochem. Photobiol. Sci., 2014, 13, 820
    DOI: 10.1039/C3PP50413A

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