Development of a traceable linker containing a thiol-responsive amino acid for the enrichment and selective labelling of target proteins

Jun Yamamoto; Masaya Denda; Nami Maeda; Miku Kita; Chiaki Komiya; Tomohiro Tanaka; Wataru Nomura; Hirokazu Tamamura; Youichi Sato; Aiko Yamauchi; Akira Shigenaga; Akira Otaka

doi:10.1039/C4OB00622D

Development of a traceable linker containing a thiol-responsive amino acid for the enrichment and selective labelling of target proteins†

Jun Yamamoto,^a Masaya Denda,^a Nami Maeda,^a Miku Kita,^a Chiaki Komiya,^a Tomohiro Tanaka,^b Wataru Nomura,^b Hirokazu Tamamura,^b Youichi Sato,^a Aiko Yamauchi,^a Akira Shigenaga*^ac and Akira Otaka*^a

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* Corresponding authors

^a Institute of Health Biosciences and Graduate School of Pharmaceutical Sciences, The University of Tokushima, Shomachi, Tokushima 770-8505, Japan
E-mail: shigenaga.akira@tokushima-u.ac.jp, aotaka@tokushima-u.ac.jp

^b Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda-ku, Tokyo 101-0062, Japan

^c JST, PRESTO, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan

Abstract

A traceable linker that is potentially applicable to identification of a target protein of bioactive compounds was developed. It enabled not only thiol-induced cleavage of the linker for enrichment of the target protein but also selective labelling to pick out the target from contaminated non-target proteins for facile identification.

Organic & Biomolecular Chemistry

Development of a traceable linker containing a thiol-responsive amino acid for the enrichment and selective labelling of target proteins†

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Development of a traceable linker containing a thiol-responsive amino acid for the enrichment and selective labelling of target proteins

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