Issue 17, 2014
From the journal:

Nanoscale

Studying the mechanism of CD47–SIRPα interactions on red blood cells by single molecule force spectroscopy†

Yangang Pan,ac   Feng Wang,a   Yanhou Liu,b   Junguang Jiang,a   Yong-Guang Yang*b  and  Hongda Wang*ac  

* Corresponding authors

a State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P.R. China
E-mail: hdwang@ciac.ac.cn

b Institute of Immunology, The First Bethune Hospital Academy of Translational Medicine, Jilin University, ChangChun, China
E-mail: yongg.yang@gmail.com

c University of Chinese Academy of Sciences, Beijing 100049, P.R. China

Abstract

The interaction forces and binding kinetics between SIRPα and CD47 were investigated by single-molecule force spectroscopy (SMFS) on both fresh and experimentally aged human red blood cells (hRBCs). We found that CD47 experienced a conformation change after oxidation, which influenced the interaction force and the position of the energy barrier between SIRPα and CD47. Our results are significant for understanding the mechanism of phagocytosis of red blood cells at the single molecule level.

Graphical abstract: Studying the mechanism of CD47–SIRPα interactions on red blood cells by single molecule force spectroscopy

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Article information

DOI
https://doi.org/10.1039/C4NR02889A
Article type
Communication
Submitted
26 May 2014
Accepted
27 Jun 2014
First published
03 Jul 2014

Nanoscale, 2014,6, 9951-9954

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Studying the mechanism of CD47–SIRPα interactions on red blood cells by single molecule force spectroscopy

Y. Pan, F. Wang, Y. Liu, J. Jiang, Y. Yang and H. Wang, Nanoscale, 2014, 6, 9951 DOI: 10.1039/C4NR02889A

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