Issue 17, 2014

Studying the mechanism of CD47–SIRPα interactions on red blood cells by single molecule force spectroscopy

Abstract

The interaction forces and binding kinetics between SIRPα and CD47 were investigated by single-molecule force spectroscopy (SMFS) on both fresh and experimentally aged human red blood cells (hRBCs). We found that CD47 experienced a conformation change after oxidation, which influenced the interaction force and the position of the energy barrier between SIRPα and CD47. Our results are significant for understanding the mechanism of phagocytosis of red blood cells at the single molecule level.

Graphical abstract: Studying the mechanism of CD47–SIRPα interactions on red blood cells by single molecule force spectroscopy

Supplementary files

Article information

Article type
Communication
Submitted
26 May 2014
Accepted
27 Jun 2014
First published
03 Jul 2014

Nanoscale, 2014,6, 9951-9954

Author version available

Studying the mechanism of CD47–SIRPα interactions on red blood cells by single molecule force spectroscopy

Y. Pan, F. Wang, Y. Liu, J. Jiang, Y. Yang and H. Wang, Nanoscale, 2014, 6, 9951 DOI: 10.1039/C4NR02889A

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