Issue 4, 2014

Programmed assembly of polymer–DNA conjugate nanoparticles with optical readout and sequence-specific activation of biorecognition

Abstract

Soft micellar nanoparticles can be prepared from DNA conjugates designed to assemble via base pairing such that strands containing a polymer corona and a cholesterol tail generate controlled supramolecular architecture. Functionalization of one DNA conjugate strand with a biorecognition ligand results in shielding of the ligand when in the micelle, while encoding of the DNA sequences with overhangs allows supramolecular unpacking by addition of a complementary strand and sequence-specific unshielding of the ligand. The molecular assembly/disassembly and ‘on–off’ switch of the recognition signal is visualized by FRET pair signalling, PAGE and a facile turbidimetric binding assay, allowing direct and amplified readout of nucleic acid sequence recognition.

Graphical abstract: Programmed assembly of polymer–DNA conjugate nanoparticles with optical readout and sequence-specific activation of biorecognition

Supplementary files

Article information

Article type
Paper
Submitted
16 Sep 2013
Accepted
04 Nov 2013
First published
05 Nov 2013
This article is Open Access
Creative Commons BY license

Nanoscale, 2014,6, 2368-2374

Programmed assembly of polymer–DNA conjugate nanoparticles with optical readout and sequence-specific activation of biorecognition

J. P. Magnusson, F. Fernández-Trillo, G. Sicilia, S. G. Spain and C. Alexander, Nanoscale, 2014, 6, 2368 DOI: 10.1039/C3NR04952C

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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