Facile protocols for the configurational assignment of primary amines and hydroxy acids by NMR

Abstract

Three-component chiral derivatization protocols are proposed for the assignment of the absolute configurations of chiral primary amines and chiral hydroxy acids using ¹H-NMR. The protocols involve simple mixing of the ternary components in CDCl₃, followed by stirring for 15 min. The spectra can be recorded directly, without invoking any separation method, unlike many other chiral derivatizing agents. The protocols permit the analysis in less than 15 min, making them convenient and effective for the assignment of the absolute configurations of primary amines and hydroxy acids.