Issue 1, 2014

Sucrose laurate-enhanced transcutaneous immunization with a solid-in-oil nanodispersion

Abstract

A novel transcutaneous immunization system was developed using a solid-in-oil (S/O) nanodispersion, consisting of a nano-sized particle of a protein–surfactant complex dispersed in an oil vehicle. Permeability of the model antigen protein, ovalbumin, was enhanced by coating with sucrose laurate, which is a hydrophobic surfactant. The nanodispersion prepared with sucrose laurate induced a 5-fold increase in mouse antigen-specific antibody production compared with that observed using sucrose erucate, indicating that stability of the S/O nanodispersion in the epidermis plays a key role in enabling effective immunization. In spite of the coating with the surfactants, only fluorescence-labeled ovalbumin permeated the deep epidermis beneath the stratum corneum and was observed by laser scanning confocal microscopy. These findings will lead the way to an improved transcutaneous immunization system based on S/O nanodispersion.

Graphical abstract: Sucrose laurate-enhanced transcutaneous immunization with a solid-in-oil nanodispersion

Supplementary files

Article information

Article type
Concise Article
Submitted
12 Jun 2013
Accepted
04 Oct 2013
First published
08 Oct 2013

Med. Chem. Commun., 2014,5, 20-24

Sucrose laurate-enhanced transcutaneous immunization with a solid-in-oil nanodispersion

M. Kitaoka, K. Imamura, Y. Hirakawa, Y. Tahara, N. Kamiya and M. Goto, Med. Chem. Commun., 2014, 5, 20 DOI: 10.1039/C3MD00164D

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