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Issue 23, 2014
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Flow-scanning optical tomography

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Abstract

We present a 3D tomography technique for in vivo observation of microscopic samples. The method combines flow in a microfluidic channel, illumination through a slit aperture, and a Fourier lens for simultaneous acquisition of multiple perspective angles in the phase-space domain. The technique is non-invasive and naturally robust to parasitic sample motion. 3D absorption is retrieved using standard back-projection algorithms, here a limited-domain inverse radon transform. Simultaneously, 3D differential phase contrast images are obtained by computational refocusing and comparison of complementary illumination angles. We implement the technique on a modified glass slide which can be mounted directly on existing optical microscopes. We demonstrate both amplitude and phase tomography on live, freely swimming C. elegans nematodes.

Graphical abstract: Flow-scanning optical tomography

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Submitted
16 Jun 2014
Accepted
07 Aug 2014
First published
07 Aug 2014

Lab Chip, 2014,14, 4447-4450
Article type
Communication
Author version available

Flow-scanning optical tomography

N. C. Pégard, M. L. Toth, M. Driscoll and J. W. Fleischer, Lab Chip, 2014, 14, 4447
DOI: 10.1039/C4LC00701H

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