Jump to main content
Jump to site search

Issue 22, 2014
Previous Article Next Article

An integrated sample-in-answer-out microfluidic chip for rapid human identification by STR analysis

Author affiliations

Abstract

A fully integrated microfluidic chip for human identification by short tandem repeat (STR) analysis that includes a unique enzymatic liquid preparation of the DNA, microliter non-contact PCR, and a polymer that allows a high-resolution separation within a compact microchip footprint has been developed. A heat-activated enzyme that digests biological materials is employed to generate the target yield of DNA from a buccal swab or FTA paper. The microfluidic architecture meters an aliquot of the liberated DNA and mixes it with the PCR reagents prior to non-contact IR-mediated PCR amplification. The products of PCR amplification are mixed with a sizing standard (ladder) and the 18-plex STR amplicons are separated in an effective length (Leff) of just 7 cm. The development, optimization and integration of each of these processes within the microfluidic chip are described. The device is able to generate genetic profiles in approximately 2 hours that match the profiles from the conventional processes performed using separate conventional instruments. Analysis is performed on a single plastic microchip with a size similar to that of a 96-well plate and only a few mm thick with no pretreatment of any of the functional domains. This is significant advancement in terms of ease of fabrication over glass microdevices or polymeric systems assembled from multiple components. Consequently, this fully integrated sample-in-answer-out microchip is an important step toward generation of a rapid micro-total analysis system for point-of-collection human identification based on genetic analysis.

Graphical abstract: An integrated sample-in-answer-out microfluidic chip for rapid human identification by STR analysis

Back to tab navigation

Supplementary files

Article information


Submitted
11 Jun 2014
Accepted
01 Sep 2014
First published
02 Sep 2014

Lab Chip, 2014,14, 4415-4425
Article type
Paper

An integrated sample-in-answer-out microfluidic chip for rapid human identification by STR analysis

D. Le Roux, B. E. Root, J. A. Hickey, O. N. Scott, A. Tsuei, J. Li, D. J. Saul, L. Chassagne, J. P. Landers and P. de Mazancourt, Lab Chip, 2014, 14, 4415
DOI: 10.1039/C4LC00685B

Social activity

Search articles by author

Spotlight

Advertisements