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Issue 13, 2014
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Phaseguide assisted liquid lamination for magnetic particle-based assays

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Abstract

We have developed a magnetic particle-based assay platform in which functionalised magnetic particles are transferred sequentially through laminated volumes of reagents and washing buffers. Lamination of aqueous liquids is achieved via the use of phaseguide technology; microstructures that control the advancing air–liquid interface of solutions as they enter a microfluidic chamber. This allows manual filling of the device, eliminating the need for external pumping systems, and preparation of the system requires only a few minutes. Here, we apply the platform to two on-chip strategies: (i) a one-step streptavidin–biotin binding assay, and (ii) a two-step C-reactive protein immunoassay. With these, we demonstrate how condensing multiple reaction and washing processes into a single step significantly reduces procedural times, with both assay procedures requiring less than 8 seconds.

Graphical abstract: Phaseguide assisted liquid lamination for magnetic particle-based assays

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Supplementary files

Article information


Submitted
31 Jan 2014
Accepted
25 Apr 2014
First published
25 Apr 2014

This article is Open Access

Lab Chip, 2014,14, 2334-2343
Article type
Paper
Author version available

Phaseguide assisted liquid lamination for magnetic particle-based assays

C. Phurimsak, E. Yildirim, M. D. Tarn, S. J. Trietsch, T. Hankemeier, N. Pamme and P. Vulto, Lab Chip, 2014, 14, 2334
DOI: 10.1039/C4LC00139G

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