Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 27th March 2019 from 11:00 AM to 1:00 PM (GMT).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 15, 2014
Previous Article Next Article

DNA-functionalized hydrogels for confined membrane-free in vitro transcription/translation

Author affiliations

Abstract

We microfluidically fabricate bio-orthogonal DNA-functionalized porous hydrogels from hyaluronic acid that are employed in in vitro transcription/translation (IVTT) of a green fluorescent protein. By co-encapsulating individual hydrogel particles and the IVTT machinery in water-in-oil microdroplets, we study protein expression in a defined reaction volume. Our approach enables precise control over protein expression rates by gene dosage. We show that gene transcription and translation are confined to the membrane-free hydrogel matrix, which contributes to the design of membrane-free protocells.

Graphical abstract: DNA-functionalized hydrogels for confined membrane-free in vitro transcription/translation

Back to tab navigation

Supplementary files

Publication details

The article was received on 21 Dec 2013, accepted on 17 Feb 2014 and first published on 24 Mar 2014


Article type: Paper
DOI: 10.1039/C3LC51427G
Citation: Lab Chip, 2014,14, 2651-2656

  •   Request permissions

    DNA-functionalized hydrogels for confined membrane-free in vitro transcription/translation

    J. Thiele, Y. Ma, D. Foschepoth, M. M. K. Hansen, C. Steffen, H. A. Heus and W. T. S. Huck, Lab Chip, 2014, 14, 2651
    DOI: 10.1039/C3LC51427G

Search articles by author

Spotlight

Advertisements