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Issue 15, 2014
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DNA-functionalized hydrogels for confined membrane-free in vitro transcription/translation

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Abstract

We microfluidically fabricate bio-orthogonal DNA-functionalized porous hydrogels from hyaluronic acid that are employed in in vitro transcription/translation (IVTT) of a green fluorescent protein. By co-encapsulating individual hydrogel particles and the IVTT machinery in water-in-oil microdroplets, we study protein expression in a defined reaction volume. Our approach enables precise control over protein expression rates by gene dosage. We show that gene transcription and translation are confined to the membrane-free hydrogel matrix, which contributes to the design of membrane-free protocells.

Graphical abstract: DNA-functionalized hydrogels for confined membrane-free in vitro transcription/translation

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Supplementary files

Article information


Submitted
21 Dec 2013
Accepted
17 Feb 2014
First published
24 Mar 2014

Lab Chip, 2014,14, 2651-2656
Article type
Paper

DNA-functionalized hydrogels for confined membrane-free in vitro transcription/translation

J. Thiele, Y. Ma, D. Foschepoth, M. M. K. Hansen, C. Steffen, H. A. Heus and W. T. S. Huck, Lab Chip, 2014, 14, 2651
DOI: 10.1039/C3LC51427G

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