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Issue 9, 2014
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Proof-of-principle for simple microshelter-assisted buffer exchange in laser tweezers: interaction of hypericin with single cells

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Abstract

Microshelters (i.e. thin dead-end side-arms of fluid channels) are used to aid buffer exchange in optical tweezers experiments. The basic idea is to transfer trapped objects into microshelters during the buffer exchange process. Particles “hidden” in microshelters become insensitive to extreme flow conditions in the main fluid channel, which minimizes the requirements for the applied flow system. The construction scheme of a simple microshelter system is described. The concept has been tested by fluorescence measurements on hypericin interaction with trapped yeast cells in different environments.

Graphical abstract: Proof-of-principle for simple microshelter-assisted buffer exchange in laser tweezers: interaction of hypericin with single cells

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Article information


Submitted
22 Oct 2013
Accepted
07 Feb 2014
First published
10 Feb 2014

Lab Chip, 2014,14, 1579-1584
Article type
Paper

Proof-of-principle for simple microshelter-assisted buffer exchange in laser tweezers: interaction of hypericin with single cells

M. A. Omar, P. Miskovsky and G. Bánó, Lab Chip, 2014, 14, 1579
DOI: 10.1039/C3LC51199E

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