Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 23, 2014
Previous Article Next Article

Pressure stabilizer for reproducible picoinjection in droplet microfluidic systems

Author affiliations

Abstract

Picoinjection is a promising technique to add reagents into pre-formed emulsion droplets on chip however, it is sensitive to pressure fluctuation, making stable operation of the picoinjector challenging. We present a chip architecture using a simple pressure stabilizer for consistent and highly reproducible picoinjection in multi-step biochemical assays with droplets. Incorporation of the stabilizer immediately upstream of a picoinjector or a combination of injectors greatly reduces pressure fluctuations enabling reproducible and effective picoinjection in systems where the pressure varies actively during operation. We demonstrate the effectiveness of the pressure stabilizer for an integrated platform for on-demand encapsulation of bacterial cells followed by picoinjection of reagents for lysing the encapsulated cells. The pressure stabilizer was also used for picoinjection of multiple displacement amplification (MDA) reagents to achieve genomic DNA amplification of lysed bacterial cells.

Graphical abstract: Pressure stabilizer for reproducible picoinjection in droplet microfluidic systems

Back to tab navigation

Supplementary files

Article information


Submitted
15 Jul 2014
Accepted
18 Sep 2014
First published
30 Sep 2014

Lab Chip, 2014,14, 4533-4539
Article type
Paper
Author version available

Pressure stabilizer for reproducible picoinjection in droplet microfluidic systems

M. Rhee, Y. K. Light, S. Yilmaz, P. D. Adams, D. Saxena, R. J. Meagher and A. K. Singh, Lab Chip, 2014, 14, 4533
DOI: 10.1039/C4LC00823E

Social activity

Search articles by author

Spotlight

Advertisements