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Issue 4, 2014
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A quantum dot-based microfluidic multi-window platform for quantifying the biomarkers of breast cancer cells

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Abstract

Conventional molecular profiling methods using immunochemical assays have limits in terms of multiplexity and the quantification of biomarkers in investigation of cancer cells. In this paper, we demonstrate a quantum dot (QD)-based microfluidic multiple biomarker quantification (QD-MMBQ) method that enables labeling of more than eight proteins immunochemically on cell blocks within 1 h, in a quantitative manner. An internal reference, β-actin, was used as a loading control to compensate for differences in not only the cell number but also in staining quality among specimens. Furthermore, the microfluidic blocking method exhibited less nonspecific binding of QDs than the conventional static blocking method.

Graphical abstract: A quantum dot-based microfluidic multi-window platform for quantifying the biomarkers of breast cancer cells

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Supplementary files

Publication details

The article was received on 27 Oct 2013, accepted on 11 Feb 2014 and first published on 12 Feb 2014


Article type: Paper
DOI: 10.1039/C3IB40224J
Citation: Integr. Biol., 2014,6, 430-437

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