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Issue 4, 2014
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Plasma membrane recovery kinetics of a microfluidic intracellular delivery platform

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Abstract

Intracellular delivery of materials is a challenge in research and therapeutic applications. Physical methods of plasma membrane disruption have recently emerged as an approach to facilitate the delivery of a variety of macromolecules to a range of cell types. We use the microfluidic CellSqueeze delivery platform to examine the kinetics of plasma membrane recovery after disruption and its dependence on the calcium content of the surrounding buffer (recovery time ∼5 min without calcium vs. ∼30 s with calcium). Moreover, we illustrate that manipulation of the membrane repair kinetics can yield up to 5× improvement in delivery efficiency without significantly impacting cell viability. Membrane repair characteristics initially observed in HeLa cells are shown to translate to primary naïve murine T cells. Subsequent manipulation of membrane repair kinetics also enables the delivery of larger materials, such as antibodies, to these difficult to manipulate cells. This work provides insight into the membrane repair process in response to mechanical delivery and could potentially enable the development of improved delivery methods.

Graphical abstract: Plasma membrane recovery kinetics of a microfluidic intracellular delivery platform

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Publication details

The article was received on 16 Oct 2013, accepted on 25 Jan 2014 and first published on 27 Jan 2014


Article type: Technical Innovation
DOI: 10.1039/C3IB40215K
Citation: Integr. Biol., 2014,6, 470-475
  • Open access: Creative Commons BY license

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