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Issue 2, 2014
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Bacterial nucleoid structure probed by active drag and resistive pulse sensing

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Abstract

Recent biophysical approaches have provided key insights into the enthalpic and entropic forces that compact the nucleoid in the cell. Our biophysical approach combines two complementary, non-invasive and label-free techniques: a precisely timed steerable optical trap and a high throughput microcapillary Coulter counter. We demonstrate the ability of the latter technique to probe the physical properties and size of many purified nucleoids, at the individual nucleoid level. The DNA-binding protein H-NS is central to the organization of the bacterial genome. Our results show that nucleoids purified from the Δhns strain in the stationary phase expand approximately five fold more than the form observed in WT bacteria. This compaction is consistent with the role played by H-NS in regulating the nucleoid structure and the significant organizational changes that occur as the cell adapts to the stationary phase. We also study the permeability to the flow of ions and find that in the experiment nucleoids behave as solid colloids.

Graphical abstract: Bacterial nucleoid structure probed by active drag and resistive pulse sensing

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Article information


Submitted
14 Jul 2013
Accepted
21 Nov 2013
First published
22 Nov 2013

Integr. Biol., 2014,6, 184-191
Article type
Paper
Author version available

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