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Issue 8, 2014
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Blueberry polyphenols prevent cardiomyocyte death by preventing calpain activation and oxidative stress

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Abstract

The purpose of this study was to examine the efficacy of an aqueous wild blueberry extract and five wild blueberry polyphenol fractions on an in vitro model of heart disease. Adult rat cardiomyocytes were pretreated with extract and fractions, and then exposed to norepinephrine (NE). Cardiomyocyte hypertrophy, cell death, oxidative stress, apoptosis and cardiomyocyte contractile function as well as the activities of calpain, superoxide dismutase (SOD) and catalase (CAT) were measured in cardiomyocytes treated with and without NE and blueberry fraction (BF). Four of five blueberry fractions prevented cell death and cardiomyocyte hypertrophy induced by NE. Total phenolic fraction was used for all further analysis. The NE-induced increase in oxidative stress, nuclear condensation, calpain activity and lowering of SOD and CAT activities were prevented upon pretreatment with BF. Reduced contractile function was also significantly improved with BF pretreatment. Blueberry polyphenols prevent NE-induced adult cardiomyocyte hypertrophy and cell death. The protective effects of BF may be in part attributed to a reduction in calpain activity and oxidative stress.

Graphical abstract: Blueberry polyphenols prevent cardiomyocyte death by preventing calpain activation and oxidative stress

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Publication details

The article was received on 12 Nov 2013, accepted on 23 May 2014 and first published on 09 Jun 2014


Article type: Paper
DOI: 10.1039/C3FO60588D
Citation: Food Funct., 2014,5, 1785-1794

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    Blueberry polyphenols prevent cardiomyocyte death by preventing calpain activation and oxidative stress

    X. L. Louis, S. J. Thandapilly, W. Kalt, M. Vinqvist-Tymchuk, B. M. Aloud, P. Raj, L. Yu, H. Le and T. Netticadan, Food Funct., 2014, 5, 1785
    DOI: 10.1039/C3FO60588D

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