A homodimeric BODIPY rotor as a fluorescent viscosity sensor for membrane-mimicking and cellular environments

Sangram Raut; Joseph Kimball; Rafal Fudala; Hung Doan; Badri Maliwal; Nirupama Sabnis; Andras Lacko; Ignacy Gryczynski; Sergei V. Dzyuba; Zygmunt Gryczynski

doi:10.1039/C4CP04260C

A homodimeric BODIPY rotor as a fluorescent viscosity sensor for membrane-mimicking and cellular environments

Sangram Raut,^a Joseph Kimball,^a Rafal Fudala,^b Hung Doan,^a Badri Maliwal,^b Nirupama Sabnis,^c Andras Lacko,^c Ignacy Gryczynski,^b Sergei V. Dzyuba^d and Zygmunt Gryczynski*^ab

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^a Department of Physics and Astronomy, Texas Christian University, Fort Worth, TX, USA
E-mail: z.gryczynski@tcu.edu

^b Department of Cell Biology and Immunology, UNT Health Science Center, Fort Worth, TX, USA

^c Department of Integrative Physiology and Anatomy, UNT Health Science Center, Fort Worth, TX, USA

^d Department of Chemistry, Texas Christian University, Fort Worth, TX, USA

Abstract

Fluorescence properties of a novel homodimeric BODIPY dye rotor for Fluorescence Lifetime Imaging Microscopy (FLIM) are reported. Steady state and time resolved fluorescence measurements established the viscosity dependent behaviour in vitro. Homodimeric BODIPY embedded in different membrane mimicking lipid vesicles (DPPC, POPC and POPC plus cholesterol) is demonstrated to be a viable sensor for fluorescence lifetime based viscosity measurements. Moreover, SKOV3 cells readily endocytosed the dye, which accumulated in membranous structures inside the cytoplasm thereby allowing viscosity mapping of internal cell components.

Physical Chemistry Chemical Physics

A homodimeric BODIPY rotor as a fluorescent viscosity sensor for membrane-mimicking and cellular environments

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A homodimeric BODIPY rotor as a fluorescent viscosity sensor for membrane-mimicking and cellular environments

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