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Issue 22, 2014
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Complexation of DNA with ruthenium organometallic compounds: the high complexation ratio limit

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Abstract

Interactions between DNA and ruthenium organometallic compounds are studied by using visible light absorption and circular dichroism measurements. A titration technique allowing for the absolute determination of the advancement degree of the complexation, without any assumption about the number of complexation modes is developed. When DNA is in excess, complexation involves intercalation of one of the organometallic compound ligands between DNA base pairs. But, in the high complexation ratio limit, where organometallic compounds are in excess relative to the DNA base pairs, a new mode of interaction is observed, in which the organometallic compound interacts weakly with DNA. The weak interaction mode, moreover, develops when all the DNA intercalation sites are occupied. A regime is reached in which one DNA base pair is linked to more than one organometallic compound.

Graphical abstract: Complexation of DNA with ruthenium organometallic compounds: the high complexation ratio limit

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Publication details

The article was received on 18 Feb 2014, accepted on 27 Mar 2014 and first published on 28 Mar 2014


Article type: Paper
DOI: 10.1039/C4CP00726C
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Citation: Phys. Chem. Chem. Phys., 2014,16, 10491-10502
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    Complexation of DNA with ruthenium organometallic compounds: the high complexation ratio limit

    S. Despax, F. Jia, M. Pfeffer and P. Hébraud, Phys. Chem. Chem. Phys., 2014, 16, 10491
    DOI: 10.1039/C4CP00726C

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