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Issue 73, 2014
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PCR quantification of SiO2 particle uptake in cells in the ppb and ppm range via silica encapsulated DNA barcodes

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Abstract

There is a strong interest in studying the cellular uptake of silica nanoparticles, particularly at medically relevant concentrations (ppb–ppm range) to understand their toxicology. At present, uptake analysis at these exposure levels is impeded by the high silica background concentration. Here we describe the use of DNA encapsulated within silica particles as a tool to quantify silica nanoparticles in in vitro cell-uptake experiments at low concentrations (down to 10 fg cell−1).

Graphical abstract: PCR quantification of SiO2 particle uptake in cells in the ppb and ppm range via silica encapsulated DNA barcodes

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Supplementary files

Article information


Submitted
12 Jun 2014
Accepted
22 Jul 2014
First published
28 Jul 2014

Chem. Commun., 2014,50, 10707-10709
Article type
Communication
Author version available

PCR quantification of SiO2 particle uptake in cells in the ppb and ppm range via silica encapsulated DNA barcodes

M. Hoop, D. Paunescu, P. R. Stoessel, F. Eichenseher, W. J. Stark and R. N. Grass, Chem. Commun., 2014, 50, 10707
DOI: 10.1039/C4CC04480K

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