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Issue 73, 2014
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PCR quantification of SiO2 particle uptake in cells in the ppb and ppm range via silica encapsulated DNA barcodes

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Abstract

There is a strong interest in studying the cellular uptake of silica nanoparticles, particularly at medically relevant concentrations (ppb–ppm range) to understand their toxicology. At present, uptake analysis at these exposure levels is impeded by the high silica background concentration. Here we describe the use of DNA encapsulated within silica particles as a tool to quantify silica nanoparticles in in vitro cell-uptake experiments at low concentrations (down to 10 fg cell−1).

Graphical abstract: PCR quantification of SiO2 particle uptake in cells in the ppb and ppm range via silica encapsulated DNA barcodes

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Publication details

The article was received on 12 Jun 2014, accepted on 22 Jul 2014 and first published on 28 Jul 2014


Article type: Communication
DOI: 10.1039/C4CC04480K
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Citation: Chem. Commun., 2014,50, 10707-10709
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    PCR quantification of SiO2 particle uptake in cells in the ppb and ppm range via silica encapsulated DNA barcodes

    M. Hoop, D. Paunescu, P. R. Stoessel, F. Eichenseher, W. J. Stark and R. N. Grass, Chem. Commun., 2014, 50, 10707
    DOI: 10.1039/C4CC04480K

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