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Issue 36, 2014
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Sensitive detection of polynucleotide kinase using rolling circle amplification-induced chemiluminescence

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Abstract

We develop a new method for the sensitive detection of polynucleotide kinase (PNK) using rolling circle amplification-induced chemiluminescence. This method exhibits high sensitivity with a detection limit of 2.20 × 10−4 U mL−1, which is superior to most reported approaches. Moreover, this method can be used to screen both the inhibitors and the activators of PNK, and can be further applied for real sample analysis.

Graphical abstract: Sensitive detection of polynucleotide kinase using rolling circle amplification-induced chemiluminescence

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Publication details

The article was received on 12 Jan 2014, accepted on 17 Mar 2014 and first published on 17 Mar 2014


Article type: Communication
DOI: 10.1039/C4CC00256C
Citation: Chem. Commun., 2014,50, 4733-4735

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    Sensitive detection of polynucleotide kinase using rolling circle amplification-induced chemiluminescence

    W. Tang, G. Zhu and C. Zhang, Chem. Commun., 2014, 50, 4733
    DOI: 10.1039/C4CC00256C

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