Issue 97, 2014
From the journal:

Chemical Communications

A general approach for the development of fluorogenic probes suitable for no-wash imaging of kinases in live cells†

Qing Zhang,a   Hui Liua  and  Zhengying Pan*a  

* Corresponding authors

a Key Laboratory of Chemical Genomics, Key Laboratory of Structural Biology, School of Chemical Biology and Biotechnology Shenzhen Graduate School, Peking University Xili University Town, PKU Campus, F-311, Shenzhen, 518055 China
E-mail: panzy@pkusz.edu.cn

Abstract

A general approach is presented for developing small molecule-based fluorogenic probes suitable for no-wash imaging of endogenous kinases in live cells. Probe 1, including a fluorophore–quencher system, was only “turned on” upon reacting with its target kinase Btk, and disclosed Btk's cellular location in live cells without any washing.

Graphical abstract: A general approach for the development of fluorogenic probes suitable for no-wash imaging of kinases in live cells

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C4CC07429G
Article type
Communication
Submitted
20 Sep 2014
Accepted
13 Oct 2014
First published
27 Oct 2014

Chem. Commun., 2014,50, 15319-15322

Author version available

Download author version (PDF)

Permissions

Request permissions

A general approach for the development of fluorogenic probes suitable for no-wash imaging of kinases in live cells

Q. Zhang, H. Liu and Z. Pan, Chem. Commun., 2014, 50, 15319 DOI: 10.1039/C4CC07429G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements