Issue 80, 2014
From the journal:

Chemical Communications

Microarray immobilization of biomolecules using a fast trans-cyclooctene (TCO)–tetrazine reaction†

Ping Wang,ab   Zhenkun Na,c   Jiaqi Fu,c   Chelsea Y. J. Tan,c   Huatang Zhang,ab   Shao Q. Yaoc  and  Hongyan Sun*ab  

* Corresponding authors

a Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institute of City University of Hong Kong, Shenzhen, P. R. China
E-mail: hongysun@cityu.edu.hk

b Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, P. R. China

c Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore

Abstract

By anchoring 1,2,4,5-tetrazine-containing biomolecules onto trans-cyclooctene (TCO)-functionalized slides, a site-specific microarray immobilization approach is described in this study. Compared with existing immobilization methods, our approach offers several distinctive features, including fast kinetics and high chemoselectivity.

Graphical abstract: Microarray immobilization of biomolecules using a fast trans-cyclooctene (TCO)–tetrazine reaction

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Article information

DOI
https://doi.org/10.1039/C4CC03838J
Article type
Communication
Submitted
20 May 2014
Accepted
14 Jul 2014
First published
14 Jul 2014

Chem. Commun., 2014,50, 11818-11821

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Microarray immobilization of biomolecules using a fast trans-cyclooctene (TCO)–tetrazine reaction

P. Wang, Z. Na, J. Fu, C. Y. J. Tan, H. Zhang, S. Q. Yao and H. Sun, Chem. Commun., 2014, 50, 11818 DOI: 10.1039/C4CC03838J

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