N-Acylhomoserine lactones (AHLs) are quorum sensing signals produced by Gram-negative bacteria. We here report the affinity purification of AHL synthases using beads conjugated with an enzyme inhibitor, which was designed based on the catalytic intermediate acyl-SAM.
K. Kai, H. Fujii, R. Ikenaka, M. Akagawa and H. Hayashi, Chem. Commun., 2014, 50, 8586 DOI: 10.1039/C4CC03094J
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