Issue 65, 2014

A general protein purification and immobilization method on controlled porosity glass: biocatalytic applications

Abstract

A general combined purification and immobilization method to facilitate biocatalytic process development is presented. The support material, EziG™, is based on controlled porosity glass (CPG) or polymer-coated versions thereof (HybCPG) and binds protein affinity tags. Biocatalytic reactions in aqueous and organic media with seven enzymes of biocatalytic interest are shown.

Graphical abstract: A general protein purification and immobilization method on controlled porosity glass: biocatalytic applications

Supplementary files

Article information

Article type
Communication
Submitted
08 Apr 2014
Accepted
20 Jun 2014
First published
03 Jul 2014

Chem. Commun., 2014,50, 9134-9137

A general protein purification and immobilization method on controlled porosity glass: biocatalytic applications

K. Engelmark Cassimjee, M. Kadow, Y. Wikmark, M. Svedendahl Humble, M. L. Rothstein, D. M. Rothstein and J.-E. Bäckvall, Chem. Commun., 2014, 50, 9134 DOI: 10.1039/C4CC02605E

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