Hoechst tagging: a modular strategy to design synthetic fluorescent probes for live-cell nucleus imaging
We report a general strategy to create small-molecule fluorescent probes for the nucleus in living cells. Our strategy is based on the attachment of the DNA-binding Hoechst compound to a fluorophore of interest. Using this approach, simple fluorescein, BODIPY, and rhodamine dyes were readily converted to novel turn-on fluorescent nucleus-imaging probes.
- This article is part of the themed collection: In celebration of Seiji Shinkai's 70th Birthday