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Issue 38, 2014
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A rapid, site-selective and efficient route to the dual modification of DARPins

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Abstract

Designed ankyrin repeat proteins (DARPins) are valuable tools in both biochemistry and medicine. Herein we describe a rapid, simple method for the dual modification of DARPins by introduction of cysteine mutations at specific positions that results in a vast difference in their thiol nucleophilicity, allowing for clean sequential modification.

Graphical abstract: A rapid, site-selective and efficient route to the dual modification of DARPins

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Supplementary files

Article information


Submitted
03 Jan 2014
Accepted
27 Mar 2014
First published
27 Mar 2014

This article is Open Access

Chem. Commun., 2014,50, 4898-4900
Article type
Communication
Author version available

A rapid, site-selective and efficient route to the dual modification of DARPins

P. Moody, V. Chudasama, R. I. Nathani, A. Maruani, S. Martin, M. E. B. Smith and S. Caddick, Chem. Commun., 2014, 50, 4898
DOI: 10.1039/C4CC00053F

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