Issue 38, 2014
From the journal:

Chemical Communications

A rapid, site-selective and efficient route to the dual modification of DARPins†

Paul Moody,ab   Vijay Chudasama,a   Ramiz I. Nathani,a   Antoine Maruani,a   Stephen Martin,b   Mark E. B. Smitha  and  Stephen Caddick*a  

* Corresponding authors

a Department of Chemistry, University College London, 20 Gordon Street, London, UK
E-mail: vpenterprise@ucl.ac.uk
Tel: +44 (0)20 7679 7538

b MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London, UK

Abstract

Designed ankyrin repeat proteins (DARPins) are valuable tools in both biochemistry and medicine. Herein we describe a rapid, simple method for the dual modification of DARPins by introduction of cysteine mutations at specific positions that results in a vast difference in their thiol nucleophilicity, allowing for clean sequential modification.

Graphical abstract: A rapid, site-selective and efficient route to the dual modification of DARPins

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Article information

DOI
https://doi.org/10.1039/C4CC00053F
Article type
Communication
Submitted
03 Jan 2014
Accepted
27 Mar 2014
First published
27 Mar 2014
This article is Open Access
Creative Commons BY license

Chem. Commun., 2014,50, 4898-4900

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A rapid, site-selective and efficient route to the dual modification of DARPins

P. Moody, V. Chudasama, R. I. Nathani, A. Maruani, S. Martin, M. E. B. Smith and S. Caddick, Chem. Commun., 2014, 50, 4898 DOI: 10.1039/C4CC00053F

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