Issue 13, 2014
From the journal:

Chemical Communications

Quencher-free molecular beacons as probes for oligonucleotides containing CAG repeat sequences†

Ki Tae Kim,a   Rakesh N. Veedu,b   Young Jun Seoc  and  Byeang Hyean Kim*a  

* Corresponding authors

a Department of Chemistry, BK School of Molecular Science, Pohang University of Science and Technology, Pohang 790-784, South Korea
E-mail: bhkim@postech.ac.kr
Fax: +82 54-279-3399
Tel: +82 54-279-5856

b School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072, Australia
E-mail: rakesh@uq.edu.au
Tel: +61 7 3365 4611

c Department of Chemistry, Chonbuk National University, Jeonju 561-756, South Korea
E-mail: yseo@jbnu.ac.kr
Fax: +82 63-270-3408
Tel: +82-54-279-2115

Abstract

We have identified quencher-free molecular beacons that allow the sensitive probing of CAG repeat oligonucleotides, including mRNA fragments of trinucleotide repeat diseases, with significant increases in fluorescence intensity mediated by disruption of the stacking of their PyU units.

Graphical abstract: Quencher-free molecular beacons as probes for oligonucleotides containing CAG repeat sequences

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Article information

DOI
https://doi.org/10.1039/C3CC48853E
Article type
Communication
Submitted
20 Nov 2013
Accepted
02 Dec 2013
First published
03 Dec 2013

Chem. Commun., 2014,50, 1561-1563

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Quencher-free molecular beacons as probes for oligonucleotides containing CAG repeat sequences

K. T. Kim, R. N. Veedu, Y. J. Seo and B. H. Kim, Chem. Commun., 2014, 50, 1561 DOI: 10.1039/C3CC48853E

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