Hydrogel-based bioassay sheets for in vitro evaluation of contraction-dependent metabolic regulation in skeletal muscle cells

Abstract

Two types of hydrogel-based bioassay sheets were developed for in vitro evaluation of contraction-dependent metabolic regulation in skeletal muscle cells: one is an oxygen sensor sheet and the other is an immunocapture sheet for a myokine, interleukin-6 (IL-6). These soft, molecularly permeable hydrogel-based bioassay sheets were directly laminated to another hydrogel on which myotubes were micropatterned, and displayed usefully measurable changes in local oxygen consumption and IL-6 secretion of myotubes upon electrically-induced contraction.