Selective electrochemical determination of dopamine in serum in the presence of ascorbic acid and uric acid by using a CuO nanoleaf electrode

Abstract

A simple, selective, and sensitive electrochemical sensor for detecting dopamine (DA) using a copper oxide (CuO) nanoleaf electrode as an active platform was developed in this work. The CuO nanoleaf electrode was fabricated via one-step chemical oxidation of copper foil immersed in an alkaline solution with a strong oxidant. The electrode exhibits selective and sensitive DA detection in the presence of ascorbic acid and uric acid in phosphate buffered saline (pH 8.0). Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were conducted to test the electrochemical behaviour of DA in the CuO nanoleaf electrode. DPV anodic peak currents at approximately 0.20 V were enhanced by increasing DA concentrations. The developed electrochemical sensor presents two linear ranges of 1 μM to 7.5 μM and 7.5 μM to 140 μM, respectively. And the detection limit of the proposed method is 0.5 μM for DA. Applying the CuO nanoleaf electrode in actual determination of DA injection yields accurate results. Furthermore, the analytical results of DA in serum show that the developed method is a promising alternative approach for complex systems.