Determination of coccidiostat clopidol on an electropolymerized-molecularly imprinted polypyrrole polymer modified screen printed carbon electrode

Abstract

The clopidol-imprinted polypyrrole films were electrochemically prepared on screen printed carbon electrodes (SPCEs) in aqueous solutions of pyrrole, clopidol and NaClO₄ as the supporting electrolyte. The clopidol template molecules were successfully trapped in the polypyrrole (PPy) film where they created artificial recognition sites. After extraction of the template, the PPy film acted as a molecularly imprinted polymer (MIP) for the specific and selective recognition of clopidol. The clopidol-imprinted PPy films were found to selectively detect clopidol. The highest anodic signal of clopidol was obtained in a phosphate buffer solution of pH 9.0. Using differential pulse voltammetry, the calibration curve for clopidol spiked to chicken meat at MIP–SPCE was linear for a concentration range of 0.3–1.8 mg kg⁻¹. The detection limit of clopidol was found as 0.21 mg kg⁻¹ (S/N = 3). The MIP–SPCE showed a stable and reproducible response without any influence of interferents existing in real samples.