Multi-mode chromatographic evaluation of a new lysine-silica stationary phase for high-performance liquid chromatography

Abstract

A new stationary phase based on silica bonded with N-terminally protected lysine was synthesized using graft-to methodology and subsequently characterized by elemental analysis, thermogravimetric analysis, and diffuse reflectance infrared Fourier transform spectroscopy. The grafted phase was then applied to multi-mode chromatography, including effective separation of hydrophobic polyaromatic compounds in reversed-phase and polar biomolecules in hydrophilic interaction liquid chromatography.