Magnetic solid-phase extraction based on a trimethylstearylammonium bromide coated Fe3O4/SiO2 composite for determination of adriamycin hydrochloride in human plasma and urine by HPLC-FLD

Abstract

A novel trimethylstearylammonium bromide (TSAB) coated Fe₃O₄/SiO₂ magnetic nanocomposite was fabricated and used as the adsorbent for the magnetic solid-phase extraction of adriamycin hydrochloride (ADR) in human plasma and urine with high performance liquid chromatography-fluorescence detection (HPLC-FLD). The factors influencing the extraction efficiency were examined including the pH value, extraction time, elution solvent, etc. A good linearity was presented by HPLC-FLD in the range of 0.5–10.0 μg mL⁻¹ for ADR, with the correlation coefficient of 0.998 (R²). The relative standard deviation was 4.27%. The limit of detection and limit of quantitation were 5.05 and 16.82 ng mL⁻¹, respectively. The feasibility of the developed method was further validated by extraction of ADR in plasma and urine samples. The recoveries were in the range of 76.5–94.0% and 77.9–96.0% for plasma and urine samples, respectively.