Determination of sesamol in sesame oil by anion exchange solid phase extraction coupled with HPLC

Abstract

An anion exchange solid phase extraction (SPE) procedure coupled with high-performance liquid chromatography was developed for the determination of sesamol in sesame oil. Sesamol was effectively extracted from sesame oil using a Cleanert PAX (500 mg per 6 mL) anion exchange SPE column. Conditions for the SPE procedure including the type, volume, and pH of the sample loading solvent, the volume and pH of washing solution, and the type, volume, and pH of the eluent were optimized for efficient sample cleanup with excellent sesamol recovery. The calibration curve of sesamol showed excellent linearity in the range of 10–500 mg L⁻¹ with a correlation coefficient (r²) of 0.9996. The SPE-HPLC method demonstrated high reproducibility with precision values (relative standard deviations, RSDs) in the range of 1.9–8.2% and high recoveries in the range of 88.2–106.1% for spiked sesame oil samples. The limit of quantitation (S/N = 10) was found to be 5.0 mg kg⁻¹. The optimized method was successfully applied to the determination of sesamol in sesame oil samples from a local market.