Molecularly imprinted polymer solid phase extraction of fungicides from wine samples

Abstract

In order to preconcentrate iprodione fungicide in white wine samples, molecularly imprinted polymers (MIPs) specific for iprodione were synthesized using two polymerization approaches: precipitation (MIP_p) and bulk polymerization (MIP_b). A comparison of the performance of the MIPs and the corresponding non-imprinted polymers (NIPs) was conducted in batch studies. In this case, the MIP_p revealed better recognition properties toward iprodione in wine samples than the MIP_b. The MIP_p and MIP_b were then used as sorbents on solid phase extraction cartridges (MISPE_p and MISPE_b consecutively) in order to pre-concentrate iprodione from white wine samples. The optimization of the MISPE elution step was done using MIP_p and acetonitrile was shown to be the best eluting solvent. MISPE_p showed better iprodione recovery and pre-concentration factor than MISPE_b. The selectivity of the MISPE method for iprodione was evaluated in white wine samples in the presence of two other fungicides, pyrimethanil and procymidone. MISPE_p was very selective for iprodione compared with the two other fungicides. However, MISPE_b was able to preconcentrate iprodione as well as its analogues.