Jump to main content
Jump to site search

Issue 18, 2014
Previous Article Next Article

A novel microfluidic device for estimating the total phenolic/antioxidant level in honey samples using a formaldehyde/potassium permanganate chemiluminescence system

Author affiliations

Abstract

A microfluidic device has been investigated as a tool for the estimation of the total phenolic content/antioxidant level in honey using an acidic potassium permanganate chemiluminescence (KMnO4-CL) detection system. Selected phenolic antioxidants, including quercetin, catechin, gallic acid, caffeic acid and ferulic acid, produced analytically useful chemiluminescence signals, with detection limits ranging between 2.4 nmol L−1 for gallic acid and 34.0 nmol L−1 for o-coumaric acid. The parameters that affect the chemiluminescence intensities of each antioxidant were carefully optimized, including chip geometry, volume and area of the detection chip, pH, and concentrations of reagents used and flow rates. The effect of formaldehyde and other enhancers on CL signal intensity was extensively investigated. The method was applied to honey samples. Nine different honey samples exhibited total phenolic/antioxidant levels of 41.2 to 765.4 mg kg−1 with respect to gallic acid. The Folin–Ciocalteu (FC) assay results were well correlated with the chemiluminescence results. The method was found to be selective, rapid and sensitive when used to estimate the total phenolic/antioxidant level, producing good agreement with reported results for honey samples.

Graphical abstract: A novel microfluidic device for estimating the total phenolic/antioxidant level in honey samples using a formaldehyde/potassium permanganate chemiluminescence system

Back to tab navigation

Supplementary files

Publication details

The article was received on 05 Mar 2014, accepted on 07 Jun 2014 and first published on 09 Jun 2014


Article type: Paper
DOI: 10.1039/C4AY00550C
Author version
available:
Download author version (PDF)
Anal. Methods, 2014,6, 7243-7249

  •   Request permissions

    A novel microfluidic device for estimating the total phenolic/antioxidant level in honey samples using a formaldehyde/potassium permanganate chemiluminescence system

    H. A. J. Al Lawati, B. Al Haddabi and F. O. Suliman, Anal. Methods, 2014, 6, 7243
    DOI: 10.1039/C4AY00550C

Search articles by author

Spotlight

Advertisements