Jump to main content
Jump to site search

Issue 13, 2014
Previous Article Next Article

A systematic quality control method of Huangqi decoction: simultaneous determination of eleven flavonoids and seven triterpenoid saponins by UHPLC-MS

Author affiliations

Abstract

A novel method of ultra high-pressure liquid chromatography coupled with mass spectrometry (UHPLC-MS) was developed for the quantitative analysis of 18 major bioactive components from Huangqi decoction (HQD). HQD is a classic traditional Chinese medicine (TCM) commonly used to treat consumptive and chronic liver diseases. Chromatographic separation was performed on a reverse-phase C18 column for 30 min at a flow rate of 1 mL min−1. The optimum mobile phase for the gradient elution was 0.05% aqueous formic acid and acetonitrile. All of the analytes showed good linearity over the tested concentration ranges (r2 > 0.9972). The recoveries of the three concentration levels ranged from 91.14% to 106.21% with relative standard deviation (RSD) less than 4.69%. Intra- and inter-day precisions were less than 4.73% and 4.97%, respectively. Moreover, this method was successfully used to determine the content of HQD extracts in three different batches. Hence, this method could be used for the multi-component quality control of HQD.

Graphical abstract: A systematic quality control method of Huangqi decoction: simultaneous determination of eleven flavonoids and seven triterpenoid saponins by UHPLC-MS

Back to tab navigation

Publication details

The article was received on 21 Jan 2014, accepted on 13 Apr 2014 and first published on 14 Apr 2014


Article type: Paper
DOI: 10.1039/C4AY00193A
Author version
available:
Download author version (PDF)
Anal. Methods, 2014,6, 4593-4601

  •   Request permissions

    A systematic quality control method of Huangqi decoction: simultaneous determination of eleven flavonoids and seven triterpenoid saponins by UHPLC-MS

    H. Luo, J. Zhong, F. Ye, Q. Wang, Y. Ma, P. Liu, H. Zhang, M. Sun and J. Jiang, Anal. Methods, 2014, 6, 4593
    DOI: 10.1039/C4AY00193A

Search articles by author

Spotlight

Advertisements