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Issue 9, 2014
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Molecularly imprinted polymer-based, solid-phase extraction followed by liquid chromatographic determination of lacosamide in rat plasma

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Abstract

A novel sample clean-up technique, water-compatible, molecularly imprinted, solid-phase extraction (MISPE), combined with high-performance liquid chromatography (HPLC), was developed and validated for selective extraction and determination of lacosamide in rat plasma. The molecularly imprinted polymer (MIP) was synthesized using methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as a cross-linker, chloroform as a porogen, and lacosamide as a template. Its efficacy as a selective sorbent in solid-phase extraction of lacosamide and the effects of various porogenic solvents, pH, crosslinker, and amount of monomer were studied and optimized. The recovery was >98% with a detection limit of 0.03 μg mL−1 over a linear range of 0.1–100 μg mL−1.

Graphical abstract: Molecularly imprinted polymer-based, solid-phase extraction followed by liquid chromatographic determination of lacosamide in rat plasma

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Article information


Submitted
09 Dec 2013
Accepted
07 Mar 2014
First published
13 Mar 2014

Anal. Methods, 2014,6, 3101-3107
Article type
Paper

Molecularly imprinted polymer-based, solid-phase extraction followed by liquid chromatographic determination of lacosamide in rat plasma

R. N. Rao, B. Sravan, Ch. K. Reddy, S. Meena, J. Prashamsa and P. Spoorthy, Anal. Methods, 2014, 6, 3101
DOI: 10.1039/C3AY42186D

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