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Issue 7, 2014
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Determination and characterization of metabolites of scutellarin produced by human intestinal bacteria using UPLC-Q-TOF/MS

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Abstract

To investigate the metabolism of scutellarin by intestinal bacteria in vitro, two strains of bacteria including Bacteroides sp. 21 and Veillonella sp. 10 were isolated from a human intestinal bacterial mixture and studied for their abilities to transform scutellarin into various metabolites. A highly sensitive and specific ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) with MetaboLynx™ software combined with the mass defect filtering (MDF) method provided high throughput capabilities for drug metabolism studies. Four metabolites were isolated using a 1.7 μm particle size Syncronis C 18 column using a gradient elution system and identified according to the mass fragmental mechanism, MS/MS fragment ions and relevant literature. The results of this study indicated that the metabolites of scutellarin were mainly in methylated and aglycone forms.

Graphical abstract: Determination and characterization of metabolites of scutellarin produced by human intestinal bacteria using UPLC-Q-TOF/MS

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Article information


Submitted
02 Dec 2013
Accepted
23 Jan 2014
First published
23 Jan 2014

Anal. Methods, 2014,6, 2314-2319
Article type
Paper

Determination and characterization of metabolites of scutellarin produced by human intestinal bacteria using UPLC-Q-TOF/MS

J. Xu, M. Zhao, D. Qian, E. Shang, S. Jiang, J. Guo, J. Duan and L. Du, Anal. Methods, 2014, 6, 2314
DOI: 10.1039/C3AY42147C

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