Jump to main content
Jump to site search

Issue 8, 2014
Previous Article Next Article

Capillary electrophoresis of a wide range of DNA fragments in a mixed solution of hydroxyethyl cellulose

Author affiliations

Abstract

We carried out capillary electrophoresis of 0.1–10.0 kilo base pair DNA fragments in a mixed hydroxyethyl cellulose (HEC) polymer. The mixed HEC polymer was prepared with different molecular weights (Mw) (90k, 250k, 720k and 1300k). The effects of important parameters, including the ratio of the mixture composition and the concentration of the mixing polymer, on the separation performance were investigated. Results show that these parameters can not only shorten the migration time of DNA without great deterioration in resolution, but they can also decrease the viscosity of the polymer, and thus make it easy to fill the capillary. Finally, we separated φ×174-Hirc II digest and λ-EcoT14 I DNA digest with high resolution in the mixed HEC solution within 18 min.

Graphical abstract: Capillary electrophoresis of a wide range of DNA fragments in a mixed solution of hydroxyethyl cellulose

Back to tab navigation

Publication details

The article was received on 06 Nov 2013, accepted on 01 Jan 2014 and first published on 09 Jan 2014


Article type: Paper
DOI: 10.1039/C3AY41965G
Author version
available:
Download author version (PDF)
Anal. Methods, 2014,6, 2473-2477

  •   Request permissions

    Capillary electrophoresis of a wide range of DNA fragments in a mixed solution of hydroxyethyl cellulose

    Z. Li, C. Liu, Y. Yamaguchi, Y. Ni, Q. You and X. Dou, Anal. Methods, 2014, 6, 2473
    DOI: 10.1039/C3AY41965G

Search articles by author

Spotlight

Advertisements