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Issue 5, 2014
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Determination of benzo(a)pyrene and aflatoxins (B1, B2, G1, G2) in vegetable oil by GPC-HPLC-FLD

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Abstract

This study develops a gel permeation chromatography-high performance liquid chromatography-fluorescence detection (GPC-HPLC-FLD) method for determination of benzo(a)pyrene and aflatoxins (B1, B2, G1, G2) in vegetable oil. In the method, sample is extracted with ethyl acetate/cyclohexane (1 : 1, v/v), and cleaned up with the GPC. The separation of target compounds is performed on a Extend C18 column (4.6 × 250 mm, 5 μm) at 25 °C with methanol and 10 mmol L−1 ammonium acetate solution as mobile phase with gradient elution at a flow rate of 1.0 mL min−1. The injection volume was 10 μL, detection wavelengths were set at 360 nm (λex) and 440 nm (λem) at 0–23 min, and 380 nm (λex) and 408 nm (λem) at 23–35 min using FLD. The detection limits of benzo(a)pyrene and aflatoxins (B1, B2, G1, G2) were 0.5, 1.0, 1.0, 1.0 and 1.0 μg kg−1, respectively. The linear detection ranges of benzo(a)pyrene and aflatoxins (B1, B2, G1, G2) are 0.5–25.0 μg kg−1, 1.0–30.0 μg kg−1, 1.0–10.0 μg kg−1, 1.0–30.0 μg kg−1, 1.0–10.0 μg kg−1 with correlation coefficients (R2) of 0.9998, 0.9999, 0.9997, 0.9998, 0.9996, respectively. Recovery rates in vegetable oil spiked with target compounds are in the range of 82.6–101.6%, with the relative standard deviation of 4.85–9.84%. The real sample tests show that this simple and accurate method can be used for determination of benzo(a)pyrene and aflatoxins (B1, B2, G1, G2) in vegetable oil.

Graphical abstract: Determination of benzo(a)pyrene and aflatoxins (B1, B2, G1, G2) in vegetable oil by GPC-HPLC-FLD

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Publication details

The article was received on 05 Nov 2013, accepted on 11 Dec 2013 and first published on 11 Dec 2013


Article type: Paper
DOI: 10.1039/C3AY41955J
Anal. Methods, 2014,6, 1545-1549

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    Determination of benzo(a)pyrene and aflatoxins (B1, B2, G1, G2) in vegetable oil by GPC-HPLC-FLD

    H. Wang, L. Zhao, H. Yang, Q. Guo, H. Shi, H. Pan, L. Zhao and C. Qian, Anal. Methods, 2014, 6, 1545
    DOI: 10.1039/C3AY41955J

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