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Issue 11, 2014
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Determination of eleven flavonoids in chamomile and linden extracts by capillary electrophoresis

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Abstract

A sensitive, dependable, simple and rapid method based on capillary electrophoresis with diode array detection (CE-DAD) was developed for identification and determination of eleven flavonoids (apigetrin, naringin, naringenin, catechin, galangin, apigenin, luteolin, quercetin, myricetin, kaempferol, and kaempferide) in chamomile and linden flower extracts. Several parameters which influence the separation were investigated to determine the optimum conditions. At room temperature, the eleven flavonoids could be well separated within 11 min. in a 55 cm length capillary at a separation voltage of 24 kV with 40 mM borate buffer (pH 8.9). Under optimum conditions, linearity was achieved within the concentration ranges of 2.5–100.0 μM for all analytes except myricetin and luteolin (5.0–100.0 μM) with correlation coefficients ≥ 0.999. The method was successfully applied to the determination of flavonoids in plant samples such as chamomile and linden flowers with satisfactory recoveries.

Graphical abstract: Determination of eleven flavonoids in chamomile and linden extracts by capillary electrophoresis

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Publication details

The article was received on 24 Oct 2013, accepted on 12 Apr 2014 and first published on 14 Apr 2014


Article type: Paper
DOI: 10.1039/C3AY41878B
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Anal. Methods, 2014,6, 3858-3864

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    Determination of eleven flavonoids in chamomile and linden extracts by capillary electrophoresis

    S. Şanlı and C. Lunte, Anal. Methods, 2014, 6, 3858
    DOI: 10.1039/C3AY41878B

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