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Issue 2, 2014
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Rapid chemical analysis of bear bile: 5 minute separation and quantitation of bile acids using UHPLC–qTOF-MS

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Abstract

Bear bile is a precious traditional Chinese medicine containing abundant bile acids. The content of these bile acids varies significantly. In this study, a rapid and reliable method was established to simultaneously monitor major and minor bile acids, using ultra-high performance liquid chromatography coupled with quadruple time-of-flight mass spectrometry. The samples were separated on a 1.8 μm Zorbax Eclipse-Plus C18 column with acetonitrile and water (containing 4 mM of ammonium acetate) as the mobile phase. A 5 min analysis allowed the characterization of 21 steroids and the quantitation of two major constituents, taurochenodeoxycholic acid (TCDCA) and tauroursodeoxycholic acid (TUDCA). The contents of TUDCA and TCDCA in 8 commercial batches of bear bile were 39.04 ± 7.83% and 36.10 ± 9.42% (w/w; mean ± standard deviation), respectively, and the ratio of TUDCA/TCDCA ranged from 0.82 to 1.76. The simultaneous qualitative and quantitative analyses of bear bile powder was achieved, and minor steroid constituents were identified.

Graphical abstract: Rapid chemical analysis of bear bile: 5 minute separation and quantitation of bile acids using UHPLC–qTOF-MS

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Publication details

The article was received on 16 Sep 2013, accepted on 29 Oct 2013 and first published on 30 Oct 2013


Article type: Paper
DOI: 10.1039/C3AY41605D
Citation: Anal. Methods, 2014,6, 596-601

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    Rapid chemical analysis of bear bile: 5 minute separation and quantitation of bile acids using UHPLC–qTOF-MS

    X. Qiao, W. Song, X. Lin, Q. Wang, T. Bo, D. Guo, J. Liu and M. Ye, Anal. Methods, 2014, 6, 596
    DOI: 10.1039/C3AY41605D

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