Issue 1, 2014

Colorimetric screening of β-glucosidase inhibition based on gold nanocomposites

Abstract

This article presents a simple gold–cellobiose nanocomposites based colorimetric assay for the screening of β-glucosidase inhibitors. The nanocomposites are composed of gold nanoparticles (AuNPs), particular cellobiose substrates, and 6-mercapto-1-hexanol. After β-glucosidase digestion, the AuNPs become more exposed and the attractive force between AuNPs is increased by the modified 6-mercapto-1-hexanol. Consequently, the aggregation of nanocomposites and the red shift of surface plasmon absorption can be observed. The absorbance ratio at 650 nm and 520 nm (A650/A520) of nanocomposites can be used to estimate the β-glucosidase activity. This technology could serve an alternative platform for the efficient screening of β-glucosidase inhibitors. Both the inhibition effect of heavy metals and surfactants on β-glucosidase could be analyzed by the detection of β-glucosidase activity. To summarize, the goal of this technical note is to develop a simple colorimetric method for the screening of β-glucosidase inhibitors.

Graphical abstract: Colorimetric screening of β-glucosidase inhibition based on gold nanocomposites

Supplementary files

Article information

Article type
Technical Note
Submitted
22 Jul 2013
Accepted
09 Oct 2013
First published
25 Nov 2013

Anal. Methods, 2014,6, 312-315

Colorimetric screening of β-glucosidase inhibition based on gold nanocomposites

C. Lai, G. Zeng, D. Huang, M. Zhao, M. Chen, Z. Wei, C. Huang, P. Xu, N. Li, X. Li and C. Zhang, Anal. Methods, 2014, 6, 312 DOI: 10.1039/C3AY41233D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements