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Issue 12, 2014
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Acetylcholinesterase biosensor based on a gold nanoparticle–polypyrrole–reduced graphene oxide nanocomposite modified electrode for the amperometric detection of organophosphorus pesticides

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Abstract

A nanohybrid of gold nanoparticles, polypyrrole, and reduced graphene oxide sheets (named as Au–PPy–rGO) was achieved by electrochemical deposition of reduced graphene oxide with pyrrole and the introduction of gold nanoparticles. Acetylcholinesterase (AChE) was further encapsulated in a silica matrix and immobilized on the Au–PPy–rGO nanocomposite by co-deposition with (NH4)2SiF6. The presence of PPy helped to avoid the aggregation of rGO caused by van der Waals interactions between individual sheets and significantly increased the surface area of the modified electrode. The obtained Au–PPy–rGO nanocomposite not only showed excellent conductivity but also exhibited a high electrocatalytic activity and specific affinity for thiocholine, the hydrolysis product of the enzyme, and thus an improved detection sensitivity. Since AChE molecules were protected by the circumambient silica matrix, which provided a biocompatible environment and facilitated mass transport, the fabricated AChE biosensor displayed high stability and excellent activity together with a fast response to organophosphorus pesticides. Under optimum conditions, the biosensor led to the rapid and sensitive detection of paraoxon-ethyl from 1.0 nM to 5 μM with a detection limit of 0.5 nM.

Graphical abstract: Acetylcholinesterase biosensor based on a gold nanoparticle–polypyrrole–reduced graphene oxide nanocomposite modified electrode for the amperometric detection of organophosphorus pesticides

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Publication details

The article was received on 10 Jan 2014, accepted on 05 Mar 2014 and first published on 06 Mar 2014


Article type: Paper
DOI: 10.1039/C4AN00068D
Citation: Analyst, 2014,139, 3055-3060
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    Acetylcholinesterase biosensor based on a gold nanoparticle–polypyrrole–reduced graphene oxide nanocomposite modified electrode for the amperometric detection of organophosphorus pesticides

    Y. Yang, A. M. Asiri, D. Du and Y. Lin, Analyst, 2014, 139, 3055
    DOI: 10.1039/C4AN00068D

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