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Issue 3, 2014

Selective detection of complementarity-determining regions of monoclonal antibody by limiting protease access to the substrate: nano-surface and molecular-orientation limited proteolysis

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Abstract

We report a novel method for the selective proteolysis by limiting protease access to the substrate, which we have named nano-surface and molecular-orientation limited (nSMOL) proteolysis. We focus on the identification of the Fab and quantitation of antibodies. This method successfully performed limited proteolysis on the Fab.

Graphical abstract: Selective detection of complementarity-determining regions of monoclonal antibody by limiting protease access to the substrate: nano-surface and molecular-orientation limited proteolysis

Supplementary files

Article information


Submitted
11 Nov 2013
Accepted
29 Nov 2013
First published
29 Nov 2013

Analyst, 2014,139, 576-580
Article type
Communication

Selective detection of complementarity-determining regions of monoclonal antibody by limiting protease access to the substrate: nano-surface and molecular-orientation limited proteolysis

N. Iwamoto, T. Shimada, Y. Umino, C. Aoki, Y. Aoki, T. Sato, A. Hamada and H. Nakagama, Analyst, 2014, 139, 576 DOI: 10.1039/C3AN02104A

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