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Issue 21, 2014
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Modification of microfluidic paper-based devices with silica nanoparticles

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This paper describes a silica nanoparticle-modified microfluidic paper-based analytical device (μPAD) with improved color intensity and uniformity for three different enzymatic reactions with clinical relevance (lactate, glucose, and glutamate). The μPADs were produced on a Whatman grade 1 filter paper and using a CO2 laser engraver. Silica nanoparticles modified with 3-aminopropyltriethoxysilane were then added to the paper devices to facilitate the adsorption of selected enzymes and prevent the washing away effect that creates color gradients in the colorimetric measurements. According to the results herein described, the addition of silica nanoparticles yielded significant improvements in color intensity and uniformity. The resulting μPADs allowed for the detection of the three analytes in clinically relevant concentration ranges with limits of detection (LODs) of 0.63 mM, 0.50 mM, and 0.25 mM for lactate, glucose, and glutamate, respectively. An example of an analytical application has been demonstrated for the semi-quantitative detection of all three analytes in artificial urine. The results demonstrate the potential of silica nanoparticles to avoid the washing away effect and improve the color uniformity and intensity in colorimetric bioassays performed on μPADs.

Graphical abstract: Modification of microfluidic paper-based devices with silica nanoparticles

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The article was received on 25 Jun 2014, accepted on 18 Aug 2014 and first published on 20 Aug 2014

Article type: Paper
DOI: 10.1039/C4AN01147C
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Analyst, 2014,139, 5560-5567

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    Modification of microfluidic paper-based devices with silica nanoparticles

    E. Evans, E. F. Moreira Gabriel, T. E. Benavidez, W. K. Tomazelli Coltro and C. D. Garcia, Analyst, 2014, 139, 5560
    DOI: 10.1039/C4AN01147C

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