Transmission line model analysis of transcription factors binding to oligoduplexes – differentiation of the effect of single nucleotide modifications

Abstract

Advanced impedance spectroscopy analysis based on the transmission line model (TLM) is explored as a novel QCM acoustic biosensing platform for the detection of the single point mutation effect on the binding of the transcription factors (TFs) to immobilized DNA oligoduplexes and the characterization of the protein–DNA mechanical properties.