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Issue 13, 2014
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A fluorescent switch for sequentially and selectively sensing copper(II) and L-histidine in vitro and in living cells

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Abstract

Herein, we report the development of a new fluorescent switch for sequential and selective sensing of Cu2+ and L-histidine (L-His) in vitro and in living cells for the first time. In the absence of metal ions, Ac-SAACQ-Gly-Gly-Gly-Lys (FITC) (1) exhibits comparable fluorescence to that of free FITC. In the presence of metal ions, 1 selectively coordinates to Cu2+, causing its fluorescence emission to be quenched via photoinduced electron transfer. Interestingly, the as-formed 1–Cu2+ complex selectively responds to L-His among the 20 natural amino acids by turning its fluorescence on. This property of fluorescence switch of 1 was successfully applied for qualitatively and quantitatively sensing Cu2+ and L-His in vitro. Using this dual functional probe, we also sequentially imaged Cu2+ and L-His in living HepG2 cells. Our new probe 1 could be applied for not only environmental monitoring but also biomolecule detection in the near future.

Graphical abstract: A fluorescent switch for sequentially and selectively sensing copper(ii) and l-histidine in vitro and in living cells

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The article was received on 27 Feb 2014, accepted on 16 Apr 2014 and first published on 17 Apr 2014


Article type: Communication
DOI: 10.1039/C4AN00410H
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Citation: Analyst, 2014,139, 3360-3364
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    A fluorescent switch for sequentially and selectively sensing copper(II) and L-histidine in vitro and in living cells

    X. Wang, Q. Miao, T. Song, Q. Yuan, J. Gao and G. Liang, Analyst, 2014, 139, 3360
    DOI: 10.1039/C4AN00410H

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