Issue 14, 2014
From the journal:

Analyst

Microarray-based resonance light scattering assay for detecting DNA methylation and human DNA methyltransferase simultaneously with high sensitivity†

Lan Ma,ab   Min Su,ab   Tao Lia  and  Zhenxin Wang*a  

* Corresponding authors

a State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, P. R. China
E-mail: wangzx@ciac.ac.cn
Fax: +86 431-85262243
Tel: +86 431-85262243

b University of Chinese Academy of Sciences, No.19A Yuquan Road, Beijing, P. R. China

Abstract

A microarray-based resonance light scattering assay, with the combination of methylation-sensitive endonuclease and gold nanoparticle (GNP) probes, has been proposed to sensitively distinguish the DNA methylation level as low as 0.01% (10 pM methylated DNA in 100 nM total DNA) and detect human DNA methyltransferase 1 (Dnmt1) down to 0.1 U mL−1.

Graphical abstract: Microarray-based resonance light scattering assay for detecting DNA methylation and human DNA methyltransferase simultaneously with high sensitivity

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Article information

DOI
https://doi.org/10.1039/C4AN00336E
Article type
Communication
Submitted
17 Feb 2014
Accepted
01 May 2014
First published
01 May 2014

Analyst, 2014,139, 3537-3540

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Microarray-based resonance light scattering assay for detecting DNA methylation and human DNA methyltransferase simultaneously with high sensitivity

L. Ma, M. Su, T. Li and Z. Wang, Analyst, 2014, 139, 3537 DOI: 10.1039/C4AN00336E

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