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Issue 10, 2014
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Imaging mass spectrometry of a mouse brain by tapping-mode scanning probe electrospray ionization

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Abstract

Methods for ambient sampling and ionization enable chemical information to be obtained with minimal sample preparation. Also, imaging mass spectrometry (IMS) enables the spatial distribution of multiple components to be determined by a single measurement. Here, we report an improved method of tapping-mode scanning probe electrospray ionization (t-SPESI) for ambient sampling and ionization in which probe oscillation is stabilized by using a piezo actuator. We demonstrate negative-mode IMS of a mouse coronal brain section and show that, compared with desorption electrospray ionization, t-SPESI provides unique features in the mass spectra: signal enhancement of fatty acid and lipids, and formation of multivalent ions tentatively assigned to gangliosides. These results would indicate the capability for the generation of multiple types of ions with t-SPESI.

Graphical abstract: Imaging mass spectrometry of a mouse brain by tapping-mode scanning probe electrospray ionization

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Article information


Submitted
18 Dec 2013
Accepted
27 Feb 2014
First published
27 Feb 2014

Analyst, 2014,139, 2336-2341
Article type
Communication

Imaging mass spectrometry of a mouse brain by tapping-mode scanning probe electrospray ionization

Y. Otsuka, J. Naito, S. Satoh, M. Kyogaku, H. Hashimoto and R. Arakawa, Analyst, 2014, 139, 2336
DOI: 10.1039/C3AN02340K

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